The CD4 antibody recognizes the human CD4 antigen which is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC Class II antigen.
The CD4 antibodies can be used for evaluating the transfection efficiency of cells transfected with pMACS 4.1 or pMACS 4-IRES when using the MACSelect™ 4 Transfected Cell Selection Kit.
Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 (VIT4) antibodies conjugated to (A) FITC, (B) PE, (C) APC, (D) VioBlue, (E) VioGreen, (F) PerCP, (G) PE-Vio770, (H) APC-Vio770, (I) PerCP-Vio700 and (J) Biotin and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD4 (VIT4)-Biotin were stained with Anti-Biotin-PE (# 130-090-756) as well. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD4 (VIT4)-PE-Vio770, CD4 (VIT4)-APC-Vio770 and CD4 (VIT4)-PerCP-Vio700.