Products and Services - MACS Cell Separation - Cell separation reagents - Tumor cells - CD326 (EpCAM) MicroBeads, human

CD326 (EpCAM) MicroBeads, human

Overview

CD326 (EpCAM) MicroBeads can be used for the:
  • positive selection or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
  • enrichment of circulating or disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples1 of patients with carcinomas,
  • positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver tissue2,3,
  • positive selection of tumor exosomes from peripheral blood4,
  • enrichment of exfoliated epithelial cells from gastric aspirates and stool samples of preterm infants5,
  • purification of primary tumor cell cultures6.

Details

Background information

CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) expression is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells7, circulating tumor cells, and cancer stem cells.

Downstream applications

Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured8 and analyzed by immunocytochemistry9, flow cytometry, or molecular biology techniques, for example, RT-PCR10.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

Figure 1
CD326 (EpCAM)+ tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.
CD326 (EpCAM)+ tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.

Figure 2

Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads.
Cell mixture before separation (6×107 PBMCs were mixed with 50,000 tumor cells)
Positive fraction with enriched breast cancer cells (1.54×105 total cells)

Related Items

Library

Selected references

  1. Lewin et al. (2006) Cancer Res. 66: 1859–1865.
  2. Schmelzer et al. (2006) Stem Cells 24: 1852–1858.
  3. Schmelzer et al. (2007) J. Exp. Med. 204: 1973–1987.
  4. Taylor et al. (2008) Gynecol. Oncol. 110: 13–21.
  5. Kaeffer et al. (2007) Pediatr. Res. 62: 564–569.
  6. Chan et al. (2006) Clin. Cancer Res. 12: 1859–1867.
  7. Moldenhauer, G. et al. (1987) Epithelium specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56: 714–721.
  8. Leinung et al. (2000) Langenbecks Arch. Surg. 385: 337–343.
  9. Krüger et al. (2000) Transfusion 40: 1489–1493.
  10. Molloy et al. (2008) Cancer Cell 112: 297–307.

Brochures and posters

Cancer Research Brochure [PDF, 4.3 MB, EN]

Product Order no. Price

CD326 (EpCAM) MicroBeads, human

Capacity: for 1×109 total cells
 
Data sheet
130-061-101 $610.00 cart

CD326 (EpCAM) MicroBeads + CD326 (EpCAM)-PE, human

Capacity: for 1×109 total cells
 
Data sheet
Data sheet
 
130-092-234 $835.00 cart

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
Contact overview

Product finder

Toolbox

New filling format for mouse and rat antibodies!

New filling format for mouse and rat antibodies!

Play MACS Scientific Games

Play MACS Scientific Games

Change country

Please select your country to continue