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Naive Pan T Cell Isolation Kit, human

  • Unrivaled: excellent purity and unmatched recovery
  • Time-saving: cells can be immediately used for downstream applications

Overview

The Naive Pan T Cell Isolation Kit was developed for the fast and efficient isolation of untouched naive T cells from human PBMCs.

Details

Detailed separation procedure

For depletion of memory T cells and non-T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), and CD244 antibodies. Optionally, biotinylated Anti-TCRγ/δ antibodies can be added for depletion of TCRγ/δ+ T cells. The cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. CD61 MicroBeads are added for concurrent depletion of platelets.

Downstream applications

Naive pan T cells isolated with the Naive Pan T Cell Isolation Kit can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T cell activation and in functional studies of DCs. Untouched naive pan T cells can also be used for further isolation of specific T cell subsets.

Columns

LS or autoMACS® Columns.

Gallery

Figure 1

Separation of untouched naive T cells from PBMCs by depletion of non-T cells using the Naive Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD3-VioBlue, CD197-PE and CD45RA-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.

PBMCs before separation

Untouched naive T cells

Related Items

Library

Selected references

  1. Che et al. (2010) Eur. J. Immunol. 40: 2248–2252.
  2. Gattinoni et al. (2011) Nat. Med. 17: 1290–1297.
  3. Jedema et al. (2011) Haematologica 96(8): 1204–1212.

Product Order no. Price

Naive Pan T Cell Isolation Kit, human

Capacity: for 1×109 total cells
 
Data sheet
130-097-095 $685.00 cart

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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