Products and Services - MACS Cell Separation - Cell separation reagents - Hematopoietic stem cells - CD34 MicroBead Kit, human

CD34 MicroBead Kit, human

  • High impact: tool of choice in more than 2,000 publications
  • Fast: cell isolation in less than one hour
  • Flexible: isolate target cells from peripheral blood, bone marrow, cord blood, differentiated ES or iPS cells and many more tissues

Overview

The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
  • peripheral blood (PBMCs)
  • bone marrow1
  • leukapheresis product2
  • cord blood3
  • differentiated ES and iPS cell lines4,5.

Details

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications

Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice.

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

Flow cytometric analysis of CD34+ cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
BM-MNCs before separation
Isolated CD34+ cells

Related Items

Library

Selected references

  1. Schreiber et al. (2009) Haematologica 94: 1493–1501.
  2. Jing et al. (2010) Bone Marrow Transplant. 45: 1489–1496.
  3. Ban et al. (2011) Proc. Natl. Acad. Sci. U.S.A. 108: 14234–9.
  4. Bandi and Akkina (2008) AIDS Res. Ther. 5: 1.
  5. Wang et al. (2007) Nat. Biotechnol. 25: 317–318.
  6. Loges et al. (2004) Stem Cells Dev. 13: 229–242.
  7. Peichev, M. et al. (2000) Blood 95: 952–958.
  8. Rafii, S. and Lyden, D. (2003) Nat. Med. 9: 702–712.
  9. Valgimigli, M. et al. (2004) Circulation 110: 1209–1212.
  10. Chen et al. (2011) Blood 117: 1530–1539.

Product Order no. Price

CD34 MicroBead Kit, human

Capacity: for 2×109 total cells
 
Data sheet
130-046-702 $575.00 cart

CD34 MicroBead Kit, human

Capacity: for 1×1010 total cells
 
Data sheet
130-046-703 $2,310.00 cart

CD34 MicroBead Kit, human – lyophilized

Capacity: for 2×109 total cells
 
Data sheet
130-097-047 $575.00 cart

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Fax:+1 530 745 2806
macs@miltenyibiotec.com
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