CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow or differentiated embryonic stem cell cultures1. In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. amniotic fluid-derived stem cells2.
CD117 (c-kit) is a receptor tyrosine kinase expressed on the majority of hematopoietic stem and progenitor cells, on some tissue stem cells as well as mature mast cells. Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance.
Bone marrow-derived CD117+ cells transplanted after myocardial infarction can differentiate into smooth muscle cells, myocytes, and endothelial cells in vivo3,4 .
MS, LS, XS, or autoMACS® Columns.
Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.
Isolated CD117+ cells after enrichment