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CD117 MicroBeads, mouse

Overview

CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow or differentiated embryonic stem cell cultures1. In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. amniotic fluid-derived stem cells2.

Details

Background information

CD117 (c-kit) is a receptor tyrosine kinase expressed on the majority of hematopoietic stem and progenitor cells, on some tissue stem cells as well as mature mast cells. Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance.

Downstream applications

Bone marrow-derived CD117+ cells transplanted after myocardial infarction can differentiate into smooth muscle cells, myocytes, and endothelial cells in vivo3,4 .

Columns

MS, LS, XS, or autoMACS® Columns.

Gallery

Figure 1

Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.
A: Before enrichment
B: Isolated CD117+ cells after enrichment

Related Items

Library

Selected references

  1. Verda et al. (2008) Stem Cells 26: 381–386.
  2. De Coppi et al. (2007) Nat. Biotechnol. 25: 100–106.
  3. Orlic et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98: 10344–10349.

Product Order no. Price

CD117 MicroBeads, mouse

Capacity: for 1×109 total cells
 
Data sheet
130-091-224 $520.00 cart

CD117 MicroBeads, mouse – lyophilized

Capacity: for 1×109 total cells
 
Data sheet
130-097-146 $520.00 cart

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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