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Diamond Basophil Isolation Kit, human

Overview

The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs.

Details

Background information

Basophilic granulocytes, also known as basophils, are a rare population of leukocytes with frequencies below 1% in peripheral blood. First described by Paul Ehrlich in 1879, basophils have long been considered as circulating mast cells. They express high-affinity immunoglobulin (Ig) E receptors (FcεRI). Upon receptor cross-linking by binding of antigen-IgE complexes, basophils degranulate and thereby release inflammatory mediators such as histamine and leukotriene. Thus, basophils play a major role in allergy, asthma, and immunity.1-4

Detailed separation procedure

First, non-basophils are magnetically labeled for depletion over a MACS Column. The pre-enriched non-labeled basophils in the flow-through are then positively selected via CD123 MicroBeads.

Downstream applications

  • Studies on signal requirements for basophil activation, induction of mediator synthesis and release etc.
  • Studies on the involvement of basophils in hypersensitivity reactions
  • Studies on cytokine expression by basophils

Columns

For the first magnetic separation (depletion): LS or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Gallery

Figure 1

Basophils were isolated from human PBMCs using the Diamond Basophil Isolation Kit and two MS Columns. Cells were fluorescently stained with CD123-PE and CD203c-APC and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Isolated basophils

Related Items

Library

Selected references

  1. Shiratori et al. (2005) J. Immunol. 175: 4441–4449.
  2. Sloane et al. (2004) Blood 104: 2832–2839.
  3. Falcone et al. (2000) Blood 96: 4028–4038.
  4. Karasuyama et al. (2009) Nat. Rev. Immunol. 9: 9–13.

Product Order no. Price

Diamond Basophil Isolation Kit, human

Capacity: for 2×109 total cells
 
Data sheet
130-094-434 $790.00 cart

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
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