Products and Services - MACS Cell Culture and Stimulation - Stimulation reagents - Antigens and polyclonal stimulation - CMV pp65 – Recombinant Protein

CMV pp65 – Recombinant Protein

Overview

CMV pp65 Recombinant Protein is designed for the in vitro stimulation of pp65-specific CD4+ and CD8+ T cells. The resulting secretion of effector cytokines permits the subsequent detection of pp65-specific effector/memory T cells, for example, by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

Details

Background information

Human cytomegalovirus (CMV, HCMV) is a member of the herpes virus group. Between 50% and 85% of human adults are infected with CMV. Once infected, the virus persists in the organism. In healthy individuals, the infection is asymptomatic, but in immunocompromised patients CMV can cause severe disease. The pp65 protein (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. pp65 is an immunodominant target of CD4+ as well as CD8+ T cell responses to CMV. pp65-specific T cells predominantly produce cytokines such as IFN-γ, IL-2, and TNF-α.

Downstream applications

  • Detection of pp65-specific effector/memory T cells, for example, by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Restimulation of cells for the isolation of viable pp65-specific CD4+ and CD8+ T cells, for example, using the MACS® Cytokine Secretion Assay – Cell Enrichment and Detection Kits.
  • Generation of pp65-specific CD4+ and CD8+ T cells from naive T cell populations, for example in immunotherapy or vaccination research.
  • Pulsing of antigen-presenting cells, e.g., as a model in research on antigen uptake and presentation.

Related Items

Library

Selected references

  1. Frentsch, M. et al. (2005) Nat. Med. 11: 1118–1124.

Product Order no. Price

CMV pp65 – Recombinant Protein

Capacity: for stimulation of up to 2×108 total cells
Content: 200 µL
 
Data sheet
130-091-824 $185.00 cart

CMV pp65 – Recombinant Protein

Capacity: for stimulation of up to 2×109 total cells
Content: 2×1 mL
 
Data sheet
130-091-823 $1,025.00 cart

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 530 745 2806
macs@miltenyibiotec.com
Contact overview

Product finder

Toolbox

GMP Cytokines

MACS GMP Products

Change country

Please select your country to continue