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T cells play an essential role in the immune system, as they are responsible for initiation and regulation of immune responses to pathogens. T cells are a heterogeneous cell population, comprising a multitude of subpopulations with distinct functions and phenotypes. Flow cytometry is a widely used method for cell analysis and is often used to elucidate the functions and roles of T cells in health and disease. However, the detection of antigen-specific T cells, for example, is hampered by their very low frequency.
This course provides hands-on experience for detecting and analyzing antigen-specific and non-specific human T cell subsets. Participants will be introduced to immunomagnetic pre-enrichment as a simple method enabling the reliable detection and analysis of rare cell types.
The training covers all subjects from a profound introduction via hands-on training through to comprehensive trouble-shooting.
Two different technical approaches will be shown and performed in the lab:
Course lectures and exercises include:
Laboratory practicals include:
Participants should be able to independently perform flow cytometric experiments.
Bergisch Gladbach/Cologne, Germany
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San Diego, CA, USA
No available training dates at the moment. Please check back later.
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