Optics | |
| Laser excitation | Spatially separated: 405 nm, 40 mW 488 nm, 50 mW 561 nm, 100 mW |
| Emission detectors | FSC: 561/4 nm SSC: 561/4 nm V1: 452/45 nm V2: 525/50 nm Y1: 586/15 nm Y2: 615/20 nm Y3: 661/20 nm Y4: 740 nm LP B1: 525/50 nm B2: 593–650 nm |
| Fluorescence sensitivity and resolution | MESFs (CV <5%): FITC <200 PE <100 |
| Flow cell dimensions | 200×250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
Fluidics | |
| Minimal uptake volume1 | 1 μL (25 μL recommended for volumetric counting applications) |
| Sample flow rate | 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second |
| Measurement speed2,3 | <25 minutes per 96-well plate (5 μL measurement volume per well) |
| Sample uptake | 1–450 µL |
| Maximal event rate | Up to 15,000 events/second |
Technical specifications | |
| Footprint | 385.5×284.5 mm (15.18×11.2 in) |
| Size (width×depth) Height (adjustable touchscreen) | 669×400 mm (26.34×15.75 in) 394–553.5 mm (15.51–21.79 in) |
| Size with miniSampler Plus (width×depth) | 669×500 mm (26.34×19.69 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6" LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display), 2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
Performance | |
| Sample carry-over2,4 | 0.01% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 8×8 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
| 1 At every uptake, an additional excess volume is aspirated by the instrument. The excess volumes are calibration- and process-dependent and do not exceed 10 μL for Fast, Standard, and Extended modes, and 20 μL for Screen mode. 2 Referred value indicates the average of multiple experiments and can differ for individual sample materials. 3 The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode. 4 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carry-over is defined by sum (CO-singlet count)/sum(SRC-singlet count) ×100%. | |
- MACSQuant Instrument user manual
- MACSQuantify Software 2.8 user manual
- MACSQuantify Software 2.10 user manual
- MACSQuantify Software 2.11 user manual
- MACSQuantify Software 2.13 user manual
- MACSQuantify™ Maintenance - short instructions
- MACSQuantify™ Start-up - short instructions
- MACSQuantify™ PMT calibration - short instructions
- MACSQuantify™ Multisampling - short instructions
- MACSQuantify™ Icons - short instructions
- MACSQuantify™ File management- short instructions
- MACSQuantify™ Acquisition - short instructions
- MACSQuantify™ Photomultiplier tube (PMT) calibration - short instructions
Brochures/posters
Scientific posters
App notes/customer reports
- Measurement assurance and harmonization using the MACSQuant® Analyzer in cell counting applications
- FRET Express Mode
- Analysis of hematopoietic cell differentiation using fluorescent protein reporters and multicolor flow cytometry.
- Determination of ploidy levels in plant cells - Applications for the MACSQuant® Analyzer
- Reliable, hands-free, and high-throughput cell viability assays on the MACSQuant® Flow Cytometer
Other downloads
- MACSQuant VYB specification sheet
- MACSQuant Instrument short instructions: Start up
- MACSQuant Instrument short instructions: Acquisition
- MACSQuant Instrument short instructions: Instrument settings
- MACSQuant Instrument short instructions: Calibration
- MACSQuant Instrument short instructions: Icon menu
- MACSQuant Instrument short instructions: Autolabeling and multisampling
Harmonizing the world is just an instrument setting away with MACSQuantify™ Software
- Easy assay transfer from one instrument to another for comparable, standardized results and multi-instrument alignment during inter-lab collaborations
- Express modes with predefined experiment settings as well as acquisition and analysis templates
- Automated fluorescence compensation
- Compliance with 21 CFR Part 11 for data submission to regulatory agencies
- Audit trail, analysis reports with e-signature, and user management system according to 21 CFR Part 11
Support at your fingertips
- MACSQuant Live Support for real-time assistance from our experts
- Technical and field application support for assay design, custom automation, and express mode development
- Training at Miltenyi University
- Comprehensive service options provided by our globally distributed field service teams
MACSQuant® VYB Flow Cytometer
Your benchmark for fluorescent protein flow cytometry
Order no.: 130-096-116. For research use only.
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