Name: MACSprep™ HLA B Cell Isolation Kit, human
Availability: InStock

Product data and images for MACSprep™ HLA B Cell Isolation Kit, human

Figures

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for MACSprep™ HLA B Cell Isolation Kit, human

Overview

The MACSprep™ HLA B Cell Isolation Kit allows the isolation of pure viable lymphocytes in only 20 minutes. Untouched B lymphocytes can be isolated with an easy two step protocol at room temperature. The isolation is performed directly from whole blood or spleen cell suspensions and does not require a density gradient centrifugation.

Detailed product information

Background information

CDC (complement dependent cytotoxicity) research requires the isolation of lymphocyte subpopulations. Enrichment of cells for these assays can be exceedingly time consuming or result in co-enrichment of unwanted or non-viable cells. The MACSprep™ HLA B Cell Isolation Kit allows the fast and easy isolation of B lymphocytes directly from samples without density gradient centrifugation or erythrocyte lysis.

Detailed separation procedure

The MACSprep™ HLA B Cell Isolation Kit allows the untouched isolation of human B lymphocytes from anticoagulated whole blood or spleen cell suspensions. Non-target cells are removed by immunomagnetic depletion while erythrocytes are sedimented, yielding viable untouched target cells of high purity.

Downstream applications

Isolated cells are immediately ready for downstream HLA research.

Resources for MACSprep™ HLA B Cell Isolation Kit, human

Documents and Protocols

Brochures/posters

MACSprep™ Technology - Excellence in cell preparation for diagnostic research

Brochures/posters

MACSprep™ HLA Cell Isolation Kits - Quick and simple isolation of B and T cells

Scientific posters

A simple and fast method for enrichment of lymphocyte subsets for complement-dependent cytotoxicity assays

Data and images

Data and images for MACSprep™ HLA B Cell Isolation Kit, human

Figures

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD19

⁺

B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress

^®

Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

MACSprep™ HLA B Cell Isolation Kit, human

MACSprep™ HLA B Cell Isolation Kit, human

Product data and images for MACSprep™ HLA B Cell Isolation Kit, human

Figures

Figure 1