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Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Before separation | After separation |
MACSprep™ HLA B Cell Isolation Kit, humanFigure 1Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. | MACSprep™ HLA B Cell Isolation Kit, humanFigure 1Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Before separation | After separation |
MACSprep™ HLA B Cell Isolation Kit, humanFigure 1Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. | MACSprep™ HLA B Cell Isolation Kit, humanFigure 1Untouched CD19 + B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress ® Separator. The isolated cells were fluorescently stained with CD45-VioBlue and CD19-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
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