Exosome are incubated with the antibody-coated MACSPlex Exosome Capture Beads. Subsequently or in parallel, exosomes bound to the MACSPlex Exosome Capture Beads are labeled with the MACSPlex Exosome Detection Reagents. The MACSPlex Exosome Detection Reagents can also be combined to create a cocktail comprising of MACSPlex Exosome Detection Reagent for CD9, CD63, and CD81. Consequently, sandwich complexes are formed between the MACSPlex Exosome Capture Bead, exosome, and the detection reagent (see Data and Images, Figure 1). These complexes can be analyzed based on the fluorescence characteristics of both the MACSPlex Exosome Capture Bead and the detection reagent. Positive signals indicate the presence of the respective surface epitope within the exosome population (see Data and Images, Figure 2). It is also possible to compare different exosome samples using the MACSPlex Exosome Kits allowing semi-quantitative analysis of differential surface epitopes.
Applications
The MACSPlex Exosome Kits allows for qualitative and semiquantitative analysis of 37 exosomal surface epitopes. The MACSPlex Exosome Kit has been developed for the simultaneous flow cytometric detection of 37 surface epitopes that are known to be present on different exosomes plus two isotype control beads.
