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One total adult rat heart was dissociated using the Neonatal Heart Dissociation Kit, mouse and rat in combination with the gentleMACS™ Octo Dissociator with Heaters. Subsequently, debris was depleted using the Debris Removal Solution and erythrocytes were removed according to the Red Blood Cell Lysis protocol described in the Neonatal Heart Dissociation Kit data sheet. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer based on scatter signals to demonstrate absence of debris after debris removal. |
After dissociation | After dissociation and debris removal | After dissociation, debris removal, and erythrocyte removal |
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