Name: Dead Cell Removal Kit
Availability: InStock

Product overview

The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.

Detailed product information

Applications

The Dead Cell Removal Kit has been successfully used for the following applications:

Removal of dead cells prior to MACS^® Separation of cells
Reduction of flow sorting time by elimination of dead cells
Elimination of dead cells from cell cultures
Improvement of immunocytochemical analysis of frozen cells
Preparation of viable, single-cell suspensions from tissue
Removal of dead cells from spermatozoa samples
Removal of dead cells prior to or following transfection of cells

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Product data and images

Figure 1

Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide.

A:	B:
Unseparated fraction	Dead cell-depleted fraction

Figure 2
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Figure 2

Working scheme for the Dead Cell Removal Kit.

Working scheme for the Dead Cell Removal Kit.

Scientific resources

Selected references
1. Kinoshita, N. et al. (2002) Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells. J. Immunol. 169: 6187-6192
2. Glander, H.J. et al. (2002) Deterioration of spermatozoal plasma membrane is associated with an increase of sperm lyso-phosphatidylcholines. Andrologia 34: 360-366

Product options: 1

130-090-101