How to assemble your MACSPlex Mix?
Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human, and the MACSPlex Cytokine Kit, mouse.
To put together your MACSPlex Mix, you need the following components:
- MACSPlex Cytokine Basic Kit, human and mouse or MACSPlex Mix Cytotoxic Basic Kit
- MACSPlex Cytokine 12 Standard, human, MACSPlex Mix Cytotoxic Standard or MACSPlex Cytokine 10 Standard, mouse
- and a combination of up to 7 individually available MACSPlex Reagent Kits (see product list below) belonging to one application category (either Human Cytokine , Human Cytotoxic T/NK or Mouse Cytokine)
Available options
Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human and the MACSPlex Cytokine Kit, mouse cannot be mixed within a single experiment.
Overview of combinable reagents and necessary software versions to run Express Modes
| Application category | Available target analytes | Standard | Basic Kit |
| Human Cytokine 12 | IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α | MACSPlex Cytokine 12 Standard, human (130-106-197) | MACSPlex Cytokine Basic Kit, human and mouse (130-109-701) |
| Mouse Cytokine 10 | IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α | MACSPlex Cytokine 10 Standard, mouse (130-106-198) | |
| Human Cytotoxic T/NK Cell | IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin | MACSPlex Mix Cytotoxic Standard (130-125-766) | MACSPlex Mix Cytotoxic Basic Kit (130-125-767) |
Basic principle
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Prerequisites
For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.
Analysis with MACSQuant Analyzers and Express Modes
ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.
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