Principle of MACSPlex Tc/NK Cell Assays.
Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Two detection reagents, composed of a cocktail of either biotin-conjugated or APC-conjugated antibodies specific for the analytes, are added.
Consequently, sandwich complexes are formed between the MACSPlex Capture Beads, the analyte and the Detection Reagent Biotin while the Detection Reagent APC binds to the biotinylated detection antibodies. These complexes can be analyzed based on the fluorescence characteristics. Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the analytes within the unknown samples.