MACSPlex Cytotoxic T/NK Cell Kit, human

MACSPlex Cytotoxic T/NK Cell Kit, human

MACSPlex assays are designed for determining concentrations of soluble analytes in a single sample. The analysis is based on MACSPlex Capture Beads which contain a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody reacting with one of the respective analytes within the sample. In combination with the Express Modes of the MACSQuant
®
Analyzers the MACSPlex Cytotoxic T/NK Cell Kit is optimized for automated measurement of beads. They simplify flow cytometric analysis via pre-defined experiment settings as well as acquisition and analysis templates. They apply an automated gating strategy to populations of interest that will be automatically adjusted for each data file individually to achieve optimal results.
The MACSPlex Cytotoxic T/NK Cell Kit, human has been developed for the simultaneous flow cytometric detection of soluble GM-CSF, Granzyme B, IFN-g, IL-2, IL-4, IL-6, IL-10, IL-17A, IL-21, MCP-1, Perforin, and TNF-a in a single sample. The kit has been optimized for use with serum, plasma, and cell culture supernatant.
MACSPlex Capture Beads consist of bead populations that have been coated with capture antibodies specific for cytokines. The bead populations can be distinguished by different fluorescence intensities detected in the B1 (FITC) and B2 (PE) channel of the MACSQuant
®
Analyzer 10 and MACSQuant X. Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Two detection reagents, one composed of a cocktail of biotin-conjugated antibodies specific for the analytes and one composed of Anti-Biotin-APC antibodes, are added. Consequently, sandwich complexes are formed between the MACSPlex Capture Bead, the analyte, and the detection reagent. These complexes can be analyzed based on the fluorescence characteristics of both the MACSPlex Capture Bead and the detection reagent. Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the analytes within the unknown samples.
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MACSPlex Cytotoxic T/NK Cell Kit, human

  • For research use only
for 100
tests
130-119-473
USD 2,420.00