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PBMCs of a CMV+
donor were stimulated for 4 hours with CMV lysate. The responding cells were stained and isolated according to secretion of TNF-α using the TNF-α Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 9390 TNF-α-secreting CD4+
T cells were enriched per 106
T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, no TNF-α-secreting CD4+
T cells were detected per 106
T cells after enrichment.
* Percentage represents frequency among CD4+ T cells.
** Percentage represents frequency among enriched cells.
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