Name: Anti-IgG1 antibodies, rat
Availability: InStock

Product overview

Clone RG11/39.4 recognizes the Fc region of the IgG1 subclass of rat immunoglobulins. Other subclasses are not detected. The antibody is suited for the flow cytometric analysis of rat B cells expressing surface IgG1, for example, memory B cells. The antibody can further be used for the indirect immunofluorescent staining and flow cytometric analysis of cells stained with primary rat antibodies of IgG1 isotype.

Alternative names

IgG1 subclass of rat immunoglobulin

Detailed product information

Technical specifications

Antigen: IgG1
Clone: RG11/39.2
Isotype: mouse IgG2aκ
Isotype control: Mouse IgG2a – isotype control antibodies
Alternative names of antigen: IgG1 subclass of rat immunoglobulin
Entrez Gene ID: 299354
Distribution of antigen: B cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: Biotin, FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, PerCP-Vio700

Product data and images

Figure 1

Splenocytes from BALB/c mice were first labeled with anti-mouse CD268 (isotype rat IgG1) and then stained with Anti-IgG1 antibodies or with the corresponding isotype control antibodies (left image), as well as with CD19 antibodies. Flow cytometry was performed with the MACSQuant

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Analyzer. Lymphocytes were pre-gated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or DAPI fluorescence, as in the case of tandem-conjugates.