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A mixture of thymocytes from C57BL/6 mice and CLEC9A transfected cells was stained with REAlease CD370 (CLEC9A)-APC (B) or with the corresponding REAL Control (A). REAlease CD370 (CLEC9A)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD370 (CLEC9A)-APC (D). Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.
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