REAlease

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CD279 (PD1) Complexes

, mouse

Technical specifications

• Antigen: CD279 (PD1)
• Clone: REAL269
• Isotype control: REAL Control
• Distribution of antigen: T cells, B cells, myeloid cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Thymocytes from BALB/c mice were stained with REAlease CD279 (PD1)-PE (B) or with the corresponding REAL Control (A). REAlease CD279 (PD1)-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD279 (PD1)-PE (D). Cells were labeled with CD3ε, CD8, and CD4 antibodies in addition. Cells were pre-gated on CD3ε + and CD8 - / dim+ cells and analysed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Thymocytes from BALB/c mice were stained with REAlease CD279 (PD1) Complexes or with the corresponding REAL Control (left images) as well as with CD3ε, CD8, and CD4 antibodies. Cells were pre-gated on CD3ε + and CD8 - / dim+ cells and analysed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Iwai, Y. et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc. Natl. Acad. Sci. U.S.A. 99: 12293-12297
  2. Salama, A. D. et al. (2003) Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J. Exp. Med. 198: 71-78
  3. Hirano, F. et al. (2005) Blockade of B7-H1 and PD-1 by monoclonal antibodies potentiates cancer therapeutic immunitiy. Cancer Res. 65: 1089-1096
  4. Tsushima, F. et al. (2007) Interaction between B7-H1 and PD-1 determines initiation and reversal of T cell anergy. Blood 110: 180-185