REAlease

^®

Anti-Siglec-F Complexes

, mouse

Technical specifications

• Antigen: Siglec-F
• Clone: REAL291
• Isotype control: REAL Control
• Alternative names of antigen: SIGLECF, SIGLEC5, mSiglec-F, Siglec-5
• Distribution of antigen: bone marrow, granulocytes, neutrophils, monocytes, eosinophils
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Bone marrow cells from C57BL/6 mice were stained with REAlease Anti-Siglec-F-APC (B) or with the corresponding REAL Control (A). REAlease Anti-Siglec-F-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Siglec-F-APC (D). Cells were labeled with CD11b antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Bone marrow cells from C57BL/6 mice were stained with REAlease Anti-Siglec-F Complexes or with the corresponding REAL Control (left images) as well as with CD11b antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Tateno, H. et al. (2005) Mouse Siglec-F and human Siglec-8 are functionally convergent paralogs that are selectively expressed on eosinophils and recognize 6-sulfo-sialyl Lewis X as a preferred glycan ligand. Glycobiology 15(11): 1125-1135
  2. Zhang, M. et al. (2007)
     Defining the

     in vivo

     function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
     Blood 109(10): 4280-4287
  3. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243