CD95 (FAS) antibodies, human

CD95 (FAS) antibodies, human

Clone: DX2 | Dilution: 1:50
CD95, also known as FAS or Apo-1, is a member of the tumor necrosis factor receptor superfamily (TNFR) and is found on the surface of many normal and neoplastically transformed cells. Its ligand, CD95L (FASL/Apo-1L), is able to induce apoptosis in CD95-expressing cells upon binding. CD95 and CD95L are up-regulated on lymphocytes upon activation and are known to play a key role in the regulation of an inflammatory response: Juxtocrine “fratricide” of neighbouring lymphocytes via mutual CD95 and CD95L expression helps to terminate immune responses, while apoptosis of pro-inflammatory cells via CD95 helps maintain immune privilege in sites such as the eye, where CD95L is found to be expressed in the retina and cornea. Cross-linking of CD95 receptors by DX2 monoclonal antibody has been described to induce apoptosis in certain target cells.

Alternative names

FAS, ALPS1A, APO-1, Apt1, FAS1, FASTM, Tnfrsf6

Technical specifications

  • Antigen: CD95 (FAS)
  • Clone: DX2
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: FAS, ALPS1A, APO-1, Apt1, FAS1, FASTM, Tnfrsf6
  • Entrez Gene ID: 355
  • Molecular mass of antigen [kDa]: 35
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: B cells, epithelial cells, fibroblasts, heart, kidney, liver, lung, monocytes, neutrophils, ovary, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, APC-Vio770, PE, PE-Vio770, FITC

  • Figure 1

    Human peripheral blood mononuclear cells (PBMCs) were stained with CD95 (FAS) antibodies as well as CD45RO and CD4 antibodies and analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  • Figure 2

    Rhesus monkey (A) or cynomolgus monkey (B) peripheral blood mononuclear cells (PBMCs) were stained with CD95 (FAS) antibodies and with CD3 antibodies and analyzed by flow cytometry.
    A:
    B:

  • Selected references

    1. Pitcher, C. J. et al. (2002)
      Development and homeostasis of T cell memory in
      rhesus macaque
      .
      J. Immunol. 168: 29-43
    2. Griffith, T. et al. (1995) Fas ligand-induced apoptosis as a mechanism of immune privilege. Science 270: 1189-1192
    3. Lynch, D. et al. (1995) Fas and FasL in the homeostatic regulation of immune responses. Immunol. Today 16: 569-574
Product options: 10
130-117-813

CD95 (FAS)-APC, human

for 30
tests
(1)
USD 124.00
130-117-701

CD95 (FAS)-APC, human

for 100
tests
(1)
USD 288.00
130-117-826

CD95 (FAS)-APC-Vio770, human

for 30
tests
(1)
USD 138.00
130-117-714

CD95 (FAS)-APC-Vio770, human

for 100
tests
(1)
USD 325.00
130-123-945

CD95 (FAS)-PE, human

for 30
tests
(1)
USD 102.00
130-123-706

CD95 (FAS)-PE, human

for 100
tests
(1)
USD 278.00
130-124-066

CD95 (FAS)-PE-Vio770, human

for 30
tests
(1)
USD 144.00
130-123-887

CD95 (FAS)-PE-Vio770, human

for 100
tests
(1)
USD 315.00
130-124-261

CD95 (FAS)-FITC, human

for 30
tests
(1)
USD 83.00
130-124-214

CD95 (FAS)-FITC, human

for 100
tests
(1)
USD 241.00

Related products

5 products available | view all

Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.

YouTube Facebook Twitter LinkedIn