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Mouse brain tissue postnatal day 6 was dissociated using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator, using a ten times lower concentration of the Solution 1. Mouse brain cells were stained with CD81 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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