Find the products and resources you are looking for!
Miltenyi Biotec distribution:
Bone marrow cells from C57BL/6 mice were stained with CD64 antibodies as well as with CD11b antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
In order to compare the epitope specificity of REAfinity Clone REA286 with other known clones, a competition assay was performed.
Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
|Other clones||Overlap in epitope recognition with REA286|
Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.