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Human peripheral blood mononuclear cells (PBMCs) were stained with CD64 antibodies or with the corresponding isotype control antibodies (left image) as well as with CD14 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem-conjugates.
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