CD63 antibodies, rat

Clone: REA444 | Dilution: 1:10
Clone REA444 recognizes the rat CD63 antigen, a multi-pass membrane protein which is also known as mast cell antigen AD1 or melanoma antigen ME491. CD63 is located on the basophilic granule membranes in resting basophils, mast cells, and platelets, and is also located on the plasma membranes of these cells. It is also found on rat embryonic fibroblasts and hepatic cell. CD63 belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, which are characterized by four membrane spanning domains. Tetraspanins, via various homotypic and heterotypic interactions, regulate the spatial location and trafficking of associated transmembrane partners, eventually regulating and co-ordinating various signaling pathways. CD63 functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. It promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading, and migration.
Additional information: Clone REA444 displays negligible binding to Fc receptors.

Alternative names

AD1, ME491

Technical specifications

  • Antigen: CD63
  • Clone: REA444
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: AD1, ME491
  • Entrez Gene ID: 29186
  • Molecular mass of antigen [kDa]: 26
  • Distribution of antigen: basophils, mast cells, platelets, fibroblasts, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, Biotin
  • Selected references

    1. Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture. J. Immunol. 149(3): 862-870
    2. Amano, T. et al. (2001) Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells. J. Biochem. 129(5): 739-744
    3. Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491. J. Biol. Chem. 266(3): 1903-1909
Product options: 8
9 µg in 300 µL (1)
USD 81.00
30 µg in 1 mL (1)
USD 168.00

CD63-PE, rat

9 µg in 300 µL (1)
USD 38.00

CD63-PE, rat

30 µg in 1 mL (1)
USD 58.00

CD63-APC, rat

9 µg in 300 µL (1)
USD 58.00

CD63-APC, rat

30 µg in 1 mL (1)
USD 78.00

CD63-Biotin, rat

9 µg in 300 µL (1)
USD 27.00

CD63-Biotin, rat

30 µg in 1 mL (1)
USD 53.00

Related products

2 products available