CD62P antibodies, human

CD62P antibodies, human

Clone: REA389 | Dilution: 1:11
Clone REA389 recognizes the human CD62P antigen, a 140 kDa single-pass type I membrane protein also known as P-selectin or platelet activation dependent granule-external membrane protein (PADGEM). CD62P is expressed by vascular endothelial cells and platelets. The protein is stored preformed in the Weibel-Palade bodies of endothelial cells and in the α-granules of platelets. Upon stimulation of these cells with a variety of agonists such as thrombin or histamine, CD62P is rapidly translocated to the cell surface, where it mediates leukocyte-platelet and leukocyte-vascular endothelial cell adhesion. The interaction of CD62P with mature leukocytes is in part responsible for mediating leukocyte rolling on vascular cells – an early event in leukocyte recruitment at sites of inflammation. In addition to its role as a receptor for mature neutrophils and monocytes, CD62P also functions as a cell adhesion molecule for leukocyte precursor cells in the bone marrow, including both lineage-restricted clonogenic progenitors and hierarchically more primitive progenitor cells.
Additional information: Clone REA389 displays negligible binding to Fc receptors.

Alternative names

SELP, CD62, GMP-140, GRMP, LECAM3, PADGEM, PSEL, P-selectin

Technical specifications

  • Antigen: CD62P
  • Clone: REA389
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: SELP, CD62, GMP-140, GRMP, LECAM3, PADGEM, PSEL, P-selectin
  • Entrez Gene ID: 6403
  • Molecular mass of antigen [kDa]: 86,2
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
  • Distribution of antigen: endothelial cells, platelets, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: Biotin

  • Figure 1

    Human peripheral blood platelets, either left unstimulated (left peak) or stimulated with thrombin, were stained with CD62P antibodies and analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. Platelets were pre-gated for the analysis based on forward and side light-scatter characteristics. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies.

  • Selected references

    1. Zannettino, A. C. et al. (1995) Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). Blood 3466(3477): 85-12
    2. Johnston, G. I. et al. (1989) Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell 56(6): 1033-1044
    3. Wickenhauser, C. et al. (2000) Selectins (CD62L, CD62P) and megakaryocytic glycoproteins (CD41a, CD42b) mediate megakaryocyte-fibroblast interactions in human bone marrow. Leuk. Res. 24(12): 1013-1021
Product options: 2
130-105-713

CD62P-Biotin, human

for 30
tests
(1)
USD 86.00
130-105-666

CD62P-Biotin, human

for 100
tests
(1)
USD 232.00

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