CD49b antibodies, mouse

CD49b antibodies, mouse

Clone: REA541 | Dilution: 1:50
Clone REA541 recognizes the mouse CD49b antigen, a single-pass type I membrane protein also known as integrin alpha-2 (ITGA2). CD49b together with CD29 (ITGB1) forms the adhesion molecule very late antigen-2 (VLA-2), which binds mainly to collagens I, II, and XI. VLA-2 is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation, and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides, and E-cadherin. Mice homozygous for a null mutation in the CD49b gene die very early in embryogenesis. CD49b is expressed on the vast majority of mouse NK cells and on a subset of NKT cells. It is not as mouse strain-restricted as other NK cell markers, for example, NK1.1, and is expressed by most common inbred mouse strains. Bone marrow (BM) resident memory T helper (Tʜ) cells also express CD49b and the homing of adoptively transferred BM memory Tʜ cells to BM can be efficiently blocked by antibodies to CD49b. This points to a decisive role of CD49b in the trafficking of memory Th cell precursors to the BM niches for memory Tʜ cells.
Additional information: Clone REA541 displays negligible binding to Fc receptors.

Alternative names

Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Technical specifications

  • Antigen: CD49b
  • Clone: REA541
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Itga2, gpIa, VLA-2α, VLA-2alpha, DX5
  • Entrez Gene ID: 16398
  • Molecular mass of antigen [kDa]: 126
  • Distribution of antigen: NK cells, T helper cells, NKT cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBright 515
  • Selected references

    1. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19(6): 739-746
    2. Hanazawa, A. et al. (2013) CD49b/CD69-dependent generation of resting T helper cell memory. Front Immunol 4: 183
    3. Edelman, J. M. et al. (1994) The mouse VLA-2 homologue supports collagen and laminin adhesion but not virus binding. Cell Adhes. Commun. 2(2): 131-143
Product options: 5
130-120-969

CD49b-PE, mouse

30 µg in 200 µL (1)
USD 55.00
130-120-885

CD49b-PE, mouse

150 µg in 1 mL (1)
USD 190.00
130-120-303

CD49b-APC, mouse

30 µg in 200 µL (1)
USD 80.00
130-120-229

CD49b-APC, mouse

150 µg in 1 mL (1)
USD 315.00
30 µg in 200 µL (1)
USD 175.00

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