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Mouse splenocytes were stained with CD43 antibodies conjugated to FITC (A), PE (B), APC (C) or Biotin (D) as well as with CD19 antibodies and analyzed by flow cytometry. Cells stained with CD43-Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.