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BALB/c splenocytes were stained with CD38 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD38-PE-Vio770.
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