Clone REA997 recognizes the human CD32 antigen, also known as FcγRII. CD32 is a 40 kDa low affinity receptor for the Fc fragment of aggregated IgG. Due to alternative splicing three different isoforms of CD32 are expressed on hematopoietic cells: FcRIIA, FcRIIB, and FcRIIC. The cytoplasmic domains of FcRIIA and FcRIIC contain an activatory ITAM motif whereas FcRIIB is an inhibitory receptor containing an ITIM motif. Different isoforms of CD32 are expressed on B cells, monocytes, dendritic cells, granulocytes, and platelets. Clone REA997 recognizes all isoforms of CD32 without blocking the binding of immune complexes.
Additional information: Clone REA997 displays negligible binding to Fc receptors.