CD304 (BDCA-4) antibodies, human

CD304 (BDCA-4) antibodies, human

Clone: REA774 | Dilution: 1:50
Clone REA774 recognizes the human CD304 (BDCA-4) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4) antigen is specifically expressed on human plasmacytoid dendritic cells in blood and bone marrow. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing. In inflamed tonsils, CD304 expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells. The CD304 (BDCA-4) is identical to neuropilin-1 (NP-1), a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells are CD304
+
but CD303 (BDCA-2)
.
Additional information: Clone REA774 displays negligible binding to Fc receptors.

Alternative names

NRP1, BDCA-4, NP1, Nrp, VEGF165R, Neuropilin-1

Technical specifications

  • Antigen: CD304 (BDCA-4)
  • Clone: REA774
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: NRP1, BDCA-4, NP1, Nrp, VEGF165R, Neuropilin-1
  • Entrez Gene ID: 8829
  • Molecular mass of antigen [kDa]: 101
  • Distribution of antigen: CNS cells, dendritic cells, endothelial cells, heart, kidney, liver, neurons, placenta, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE-Vio770, APC-Vio770, Biotin, FITC, PE, APC, PE-Vio615
  • Selected references

    1. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    2. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    3. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    4. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    5. Jongbloed, S. L. et al. (2010)
      Human CD141
      +
      (BDCA-3)
      +
      dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens.
      J. Exp. Med. 207(6): 1247-1260
    6. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
    7. Moschella, F. et al. (2013) Cyclophosphamide induces a type I interferon-associated sterile inflammatory response signature in cancer patients' blood cells: implications for cancer chemoimmunotherapy. Clin. Cancer Res. 19(15): 4249-4261
    8. Jardine, L. et al. (2013) Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment. Front Immunol 4: 495
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