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Spleen cells from C57/BL6 mice were stained with CD180 (RP105) antibodies as well as with CD45R (B220) antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. CD45.2+ cells were pre-gated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or DAPI fluorescence as in the case of tandem-conjugates.
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