CD180 (RP105) antibodies – functional grade, mouse

CD180 (RP105) antibodies – functional grade, mouse

Clone: RP/14
Clone RP/14 recognizes CD180, a type I transmembrane protein structurally similar to Toll like receptors (TLRs), with the conserved features of TLRs, including a leucine-rich repeat extracellular domain and regions containing conserved cysteines. However, in contrast to TLRs, CD180 lacks a typical TIR domain, containing only 6 to 11 intracytoplasmic amino acids. Antibody mediated ligation of CD180 was shown to induce B cell proliferation and protection from subsequent radiation- or dexamethasone-induced apoptosis. Expression of CD180 is found on mature B cells, monocytes, macrophages, and dendritic cells. CD180 activity is dependent on physical association with a molecule called MD-1. In B cells, CD180, together with TLR4, has been shown to induce B cell proliferation in response to LPS. However, in primary myeloid cells and
in vivo
, CD180 acts as an inhibitor of TLR4 signaling.

Applications

This pure – functional grade antibody is suited for cell culture functional assays.

Alternative names

Ly78, RP105

Technical specifications

  • Antigen: CD180 (RP105)
  • Clone: RP/14
  • Isotype: rat IgG2aκ
  • Alternative names of antigen: Ly78, RP105
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Miura, Y. et al. (1998) RP105 Is associated with MD-1 and transmits an activation signal in human B cells. Blood 92: 2815-2822
    2. Divanovic, S. et al. Negative regulation of Toll-like receptor 4 signaling by the Toll-like receptor homolog RP105. Nat. Immunol. 6(6): 571-578
    3. Ogata, H. et al. (2000) The toll-like receptor protein Rp105 regulates lipopolysaccharide signaling in B cells. J. Exp. Med. 192: 23-30
Product options: 1
500 µg in 0.5 mL
USD 355.00

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