CD171 (L1CAM) antibodies, mouse

CD171 (L1CAM) antibodies, mouse

Clone: 555 | Dilution: 1:10
The monoclonal antibody clone 555 recognizes CD171, a single pass type I membrane glycoprotein belonging to the immunoglobulin superfamily. Also known as L1CAM (L1 cell adhesion molecule) or NCAM-L1, CD171 belongs to a subgroup of cell adhesion molecules, which consists of six Ig-like domains, five fibronectin type III (FNIII)-like domains, a transmembrane region and a cytoplasmic domain. Expression of CD171 is found on tetanus-toxin positive neurons1, endothelial cells, certain epithelial cells, reticular fibroblasts and several malignant tumors including colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin, where a role of CD171 in augmenting tumor growth has been demonstrated. Thus, CD171 is considered a novel marker for carcinoma progression.
CD171 displays binding to a number of ligands including homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules and thus plays a vital role in cell adhesion and signal transduction. It is involved in the development of the nervous system and regulates processes such as neuron–neuron adhesion, myelination, axonal guidance, and neuronal migration.

Alternative names

L1CAM, L1, NCAM-L1

Technical specifications

  • Antigen: CD171 (L1CAM)
  • Clone: 555
  • Isotype: rat IgG2a
  • Isotype control: Rat IgG2a – isotype control antibodies
  • Alternative names of antigen: L1CAM, L1, NCAM-L1
  • Entrez Gene ID: 16728
  • Molecular mass of antigen [kDa]: 139
  • Distribution of antigen: endothelial cells, fibroblasts, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin, pure
  • Selected references

    1. Rathjen, F. G. et al. (1984) Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion. EMBO J. 3(1): 1-10
    2. Jungblut, M. et al. (2012) Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1. Glia 60(6): 894-907
    3. G. Kantzer, C. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990-1004
    4. Schaefer, A. W. et al. (1999) Activation of the MAPK signal cascade by the neural cell adhesion molecule L1 requires L1 internalization. J. Biol. Chem. 274: 37965-37973
    5. Batiuk, M. Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J. Biol. Chem. 292(21): 8874-8891
    6. Itoh, K. et al. (2004) Brain development in mice lacking L1–L1 homophilic adhesion. J. Cell Biol. 165: 145-154
    7. Needham, L. K. et al. (2001) Cytoplasmic domain mutations of the L1 cell adhesion molecule reduce L1-ankyrin interactions. J. Neurosci. 21: 1490-1500
    8. Huszar, M. et al. (2006) Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Hum. Pathol. 37(8): 1000-1008
  • Special protocols

Product options: 11
9 µg in 300 µL (1)
USD 84.00
30 µg in 1 mL (1)
USD 174.00
9 µg in 300 µL (1)
USD 41.00
30 µg in 1 mL (1)
USD 56.00
9 µg in 300 µL (1)
USD 56.00
30 µg in 1 mL (1)
USD 80.00
9 µg in 300 µL (1)
USD 47.00
30 µg in 1 mL (1)
USD 136.00
9 µg in 300 µL (1)
USD 31.00
30 µg in 1 mL (1)
USD 56.00
100 µg in 1 mL
USD 415.00

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