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Human peripheral blood cells after erythrocyte lysis were stained with CD16b antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD15 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.