CD161 antibodies, human

Technical specifications

• Antigen: CD161
• Clone: REA631
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: NKR-P1A, HNKR-P1a, CLEC5B
• Entrez Gene ID: 3820
• Molecular mass of antigen [kDa]: 25
• Distribution of antigen: liver, NK cells, T cells, thymocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin, VioBright 515, PE-Vio615

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD56 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Lanier, L. L. et al. (1994) Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428
  2. Pozo, D. et al. (2006) CD161 (human NKRP1A) signaling in NK cells involves the activation of acid sphingomyelinase. J. Immunol. 176: 2397-2406
  3. Takahashi, T. et al. (2006) Expression of CD161 (NKR-P1A) defines subsets of human CD4 and CD8 T cells with different functional activities.​ J. Immunol. 176: 211-216