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Splenocytes from C57BL/6 mice were stained with CD16/CD32 antibodies or with the corresponding isotype control antibodies (left image) without FcR Blocking Reagent. Cells were then analyzed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI).
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