CD154 antibodies, human

CD154 antibodies, human

Clone: 5C8 | Dilution: 1:10
The antibody specifically recognizes the human CD154 antigen, a 39 kDa transmembrane glycoprotein, also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4
T cells and plays an important role as a costimulatory molecule in T cell/antigen-presenting cell interactions through ligation of CD40. The antibody has been shown to block the activation of antigen-presenting cells by T helper cells
in vitro
. Because of its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4
T cells. The addition of a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154 expression induced by interaction with CD40 expressed on antigen-presenting cells. For intracellular detection of CD154 expression or if a pure population of enriched T cells is used, blocking of CD40 is not required.

Alternative names


Technical specifications

  • Antigen: CD154
  • Clone: 5C8
  • Isotype: mouse IgG2aκ
  • Isotype control: Mouse IgG2a – isotype control antibodies
  • Alternative names of antigen: CD40LG, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39
  • Entrez Gene ID: 959
  • Molecular mass of antigen [kDa]: 29
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    cynomolgus monkey (
    Macaca fascicularis
  • Distribution of antigen: basophils, dendritic cells, lymphocytes, macrophages, mast cells, monocytes, NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, Biotin
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