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Human peripheral blood mononuclear cells (PBMCs) were fixed, permeabilized, and intracellularly stained with CD152 antibodies as well as with CD25 and CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The specificity of the conjugated antibodies is confirmed by blocking the binding to ligand, using pure unconjugated antibodies (left images). Cell debris and dead cells were excluded from the analysis based on scatter signals.
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