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Human peripheral blood mononuclear cells (PBMCs), stimulated for 4 hours with LPS, were stained with CD142 antibodies as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or DAPI fluorescence, as in the case of tandem-conjugates.
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