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Bone marrow cells from BALB/c mice were stained with CD106 (VCAM-1) antibodies or with the corresponding Rat IgG2a isotype control antibodies (left image), as well as with CD11b antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.