Anti-ZAP70 pY292 antibodies, human

Anti-ZAP70 pY292 antibodies, human

Clone: REA411 | Dilution: 1:11
Clone REA411 recognizes the human Zeta-chain-associated protein kinase 70 (ZAP70) antigen phosphorylated at tyrosine 292 (pY292). ZAP70 plays an essential role in regulation of the adaptive immune response. It is normally expressed near the surface membrane of T cells and natural killer cells. ZAP70 associates with the ζ subunit of the T cell antigen receptor, and plays a critical role in T cell–signaling. It regulates both T cell–activation switch on and switch off by modulating TCR expression at the T cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection. Additionally, the ZAP70-dependent signaling pathway may also contribute to primary B cells formation and activation through the B cell receptor. Recruitment and activation of ZAP70 are transient and are terminated by phosphorylation of negative regulatory tyrosine residues and dephosphorylation of positively acting sites.
In vivo
analyses suggest that phosphorylation of residue Y292 plays a role in attenuation of TCR signaling.
Additional information: Clone REA411 displays negligible binding to Fc receptors.

Alternative names


Technical specifications

  • Antigen: ZAP70 pY292
  • Clone: REA411
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: SRK, STCD, STD, TZK, ZAP-70
  • Entrez Gene ID: 7535
  • Molecular mass of antigen [kDa]: 70
  • Distribution of antigen: T cells, NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Chan, A. C. et al. (1992) ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain. Cell 71(4): 649-662
    2. Arpaia, E. et al. (1994)
      Defective T cell receptor signaling and CD8
      thymic selection in humans lacking zap-70 kinase.
      Cell 76(5): 947-958
    3. Bottini, N. et al. (2002) Activation of ZAP-70 through specific dephosphorylation at the inhibitory Tyr-292 by the low molecular weight phosphotyrosine phosphatase (LMPTP). J. Biol. Chem. 277(27): 24220-24224
Product options: 4
for 30
USD 97.00
for 100
USD 272.00
for 30
USD 124.00
for 100
USD 288.00

Related products

4 products available