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Mouse TIM-4 transfected L5178Y cells were stained with Anti-TIM-4 antibodies or with the corresponding REA Control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.