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Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR Vβ5.3 antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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