Anti-Siglec-F antibodies, mouse

Anti-Siglec-F antibodies, mouse

Clone: ES22-10D8 | Dilution: 1:10
The monoclonal antibody ES22-10D8 recognizes mouse sialic acid-binding immunoglobulin-like lectin F (siglec-F), which is a member of the Ig superfamily containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. In mouse, siglec-F is an inhibitory receptor on eosinophils. It recognizes preferentially the glycan ligand 6'-sulfated sialyl Lewis X.

Alternative names

SIGLECF, SIGLEC5, mSiglec-F

Technical specifications

  • Antigen: Siglec-F
  • Clone: ES22-10D8
  • Isotype: rat IgG1
  • Isotype control: Rat IgG1 – isotype control antibodies
  • Alternative names of antigen: SIGLECF, SIGLEC5, mSiglec-F
  • Entrez Gene ID: 233186
  • Molecular mass of antigen [kDa]: 60
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE-Vio770, PE

  • Figure 1

    Mouse bone marrow cells were stained with Anti-Siglec-F antibodies as well as with CD11b antibodies and analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

  • Selected references

    1. Zhang, M. et al. (2007)
      Defining the
      in vivo
      function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
      Blood 109(10): 4280-4287
    2. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243
    3. Tateno, H. et al. (2005) Mouse Siglec-F and human Siglec-8 are functionally convergent paralogs that are selectively expressed on eosinophils and recognize 6-sulfo-sialyl Lewis X as a preferred glycan ligand. Glycobiology 15(11): 1125-1135
Product options: 4
130-102-274

Anti-Siglec-F-PE, mouse

30 µg in 1 mL (1)
USD 55.00
130-102-241

Anti-Siglec-F-APC, mouse

30 µg in 1 mL (1)
USD 80.00
130-103-125

Anti-Siglec-F-PE-Vio770, mouse

9 µg in 300 µL (1)
USD 52.00
130-102-167

Anti-Siglec-F-PE-Vio770, mouse

30 µg in 1 mL (1)
USD 136.00

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