Anti-S6 pS235/pS236 antibodies, human

Anti-S6 pS235/pS236 antibodies, human

Clone: REA454 | Dilution: 1:11
Clone REA454 recognizes the human 40S ribosomal protein S6 antigen phosphorylated at serine 235 and 236 (pS235/pS236). Ribosomal protein S6 is one of 33 proteins that comprise the 40 S ribosomal subunit and becomes phosphorylated at several serine residues upon mitogen stimulation. It plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Activation of the p90 ribosomal S6 kinases (RSKs) by serum, growth factors, tumor promoting phorbol esters, and oncogenic Ras is required for ribosomal protein S6 phosphorylation downstream of the Ras/ERK signaling cascade. The phosphorylation sites in ribosomal protein S6 have been mapped to five clustered serine residues – S235, S236, S240, S244, and S247 – which are located at the C terminus and are conserved from Drosophila to mammals. While ribosomal S6 kinase 1 phosphorylates ribosomal protein S6 at all sites, RSK exclusively phosphorylates ribosomal protein S6 at S235 and S236
in vitro
in vivo
using an mTOR-independent mechanism.
Additional information: Clone REA454 displays negligible binding to Fc receptors.

Alternative names

RPS6, NP33, RS6

Technical specifications

  • Antigen: S6 pS235/pS236
  • Clone: REA454
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: RPS6, NP33, RS6
  • Entrez Gene ID: 6194
  • Molecular mass of antigen [kDa]: 29
  • Distribution of antigen: other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Lott, J. B. et al. (1988) Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6. Gene 65(1): 31-39
    2. Roux, P. P. et al. (2007) RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation. J. Biol. Chem. 282(19): 14056-14064
    3. Selman, C. et al. (2009) Ribosomal protein S6 kinase 1 signaling regulates mammalian life span. Science 326(5949): 140-144
Product options: 4
for 30
USD 104.00
for 100
USD 278.00
for 30
USD 124.00
for 100
USD 288.00

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