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EA.hy926 cells, overnight serum starved and either left unstimulated (left peak) or stimulated with 200 nM Calyculin A for 30 minutes, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were stained with Anti-p120 Catenin pT310 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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