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BALB/c splenocytes were stimulated with CD3ε and CD28 functional grade antibodies over night and were stained with Anti-Notch1 antibodies and analyzed using the MACSQuant®
Analyzer. Cells labeled with Anti-Notch1-Biotin were stained with Anti-Biotin-PE in addition. The left curve in the graph shows cells from a C 4–
gate; the right line shows a gate on CD4+
cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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