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Splenocytes from C57BL/6 mice were stained with Anti-NKG2A/C/E antibodies or with the corresponding Rat IgG2a isotype control antibodies (left image), as well as with Anti-NK1.1 antibodies. Flow cytometry was performed with the MACSQuant®
cells were pregated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.